Journal: Cell reports

Article Title: Sensation and expectation are embedded in mouse motor cortical activity.

doi: 10.1016/j.celrep.2024.114396

Figure Lengend Snippet: Figure 3. M2 neurons are sound responsive (A) Example neuron’s responses to passive sounds of varying frequency. (B) Heatmap of sound-evoked response magnitudes of simultaneously recorded neurons in an example mouse. (C) T-statistic results from a linear regression incor- porating videography-captured startle movements, tone frequency, and interaction terms to predict trial- by-trial neural responses to passive sounds. (D) Schematic showing anterograde injection of AAV- TdTomato into the AC and expression of TdTomato+

Article Snippet: Tracing of connections between AC and M2 (Figures 3D and S3E–S3J) were performed using the procedure described above, with injections of anterogradely-delivered TdTomato (AAV-CAG-tdTomato, UNC, titer 5.3e12) and ACwas injectedwith retrogradely delivered GFP (pAAV-hSyn-EGFP, Addgene, titer 15e12).

Techniques: Injection, Expressing

Journal: bioRxiv

Article Title: Spontaneously regenerative corticospinal neurons in mice

doi: 10.1101/2024.09.09.612115

Figure Lengend Snippet: (A) A maximumintensity z-projection on a coronal plane of a cleared mScarlet-H2B-retro injection site expressing native mScarlet. (B) A maximum-intensity z-projection on a coronal plane of a cleared mGreenLantern-H2B-retro injection site expressing native mGreenLantern. (C) A maximum intensity z-projection on a sagittal plane of a cleared dorsal hemisection. AAV1-Cre was injected into the hindlimb cortex of an Ai14 tdT reporter mouse such that the severed CST was visible as native tdT signal (D) An image of a cleared cervical spinal cord fluorogold injection site from a coronal perspective. UV light did not penetrate the cleared tissue in a light-sheet microscope, so this image of native FG fluorescence was taken on an epifluorescence microscope. The brightness of representative images was adjusted in each channel so that tissue was visible via autofluorescence. (E) Box plots showing total fluorescent nuclei counts among all mice in both sham and hemisected groups from both fluorophores (p = 0.89 mGL and 0.43 mSc, n = 11 DHX, 13 sham, unpaired two-sided t-test). Hemisected mice are displayed in purple and sham in gray. (F) Box plots showing total FG(+) neuron counts, irrespective of whether they coincided with lumbar projecting nuclei (p = 0.33, n = 11 DHX, 13 sham, unpaired two-sided t-test). (G) A histogram shows the FG signal distribution among all retrogradely traced lumbar projecting nuclei in sham and hemisected mice. The dotted line at 55 represents the cutoff used in . (H) Heat maps showing the analysis results in for various combinations of the two threshold values. In each, the rows represent FG cutoffs from 55–75. Columns represent ipsilateral fluorophore cutoffs such that 85–95% of nuclei are designated contralateral. The two left heat maps are the results of Wilcoxon signed-rank tests of the share of FG(+) neurons in hemisected mice vs sham, in contralateral neurons (top) and ipsilateral neurons (bottom). The two right heat maps are paired Wilcoxon signed-rank test between ipsilateral and contralateral neurons in the sham mice (top) and in the hemisected mice (bottom). Asterisks denote p-values less than 0.05.

Article Snippet: To test the efficacy of the DHX procedure, pENN.AAV.hSyn.Cre.WPRE.hGH packaged in AAV1 (Addgene 105553-AAV1, RRID:Addgene_105553) at 1.9e13 vg/mL titer as injected into a 60-day-old heterozygous Ai14 tdT mouse at a rate of 100 nL/minute into the hindlimb cortex according to the previously-published , coordinates expressed relative to bregma as [mm anterior-posterior, mm medial-lateral, mm dorsal-ventral]: [-1.3, 1, -0.65].

Techniques: Injection, Expressing, Microscopy, Fluorescence